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vsv g envelope expressing plasmid pmd2 g  (Addgene inc)


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    Structured Review

    Addgene inc vsv g envelope expressing plasmid pmd2 g
    Vsv G Envelope Expressing Plasmid Pmd2 G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vsv+g+envelope+expressing+plasmid+pmd2+g/pMD2%2EG+(Plasmid+%2312259)/pm41896222-270-21-26
    Average 98 stars, based on 14751 article reviews
    vsv g envelope expressing plasmid pmd2 g - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Transfection:

    Article Title: ORMDL3 restrains type-I interferon signaling and anti-tumor immunity by promoting RIG-I degradation
    Article Snippet: .. HEK293T cells were co- transfected with lentiviral expressing plasmid, lentiviral packaging plasmid psPAX2 (Addgene, #12260), and VSV- G envelope expressing plasmid pMD2.G (Addgene, #12259). .. After 48 hr, the lentiviruses were used for infecting A549 cells and then selected cells with puromycin (Thermo Fisher Scientific).

    Article Title: Interaction between HIV-1 Tat and EBV Zta favours immune escape of B cells by downregulating HLA-ABC expression
    Article Snippet: .. HEK 293 T cells were transfected with second-generation lentiviral packaging plasmid psPAX2 (Addgene, # 12260), VSV-G envelope expressing plasmid pMD2.G (Addgene, # 12259) and transfer plasmid (FUdeltaGW-rtTA, FUW-tetO-YFP, FUW-tetO-YFP-Tat, FUW-tetO-YFP-Zta, FUW-tetO-YFP1-Tat-P2A-YFP2-Zta) at 15:6:20 ratio using Turbofect transfection reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions (20 μg of plasmid DNA and 40 μl of Turbofect transfection reagent were premixed in 2 ml of OptiMEM medium (Gibco) and added to 20 ml of DMEM/10% FBS cell medium in T-175 flask). ..

    Expressing:

    Article Title: ORMDL3 restrains type-I interferon signaling and anti-tumor immunity by promoting RIG-I degradation
    Article Snippet: .. HEK293T cells were co- transfected with lentiviral expressing plasmid, lentiviral packaging plasmid psPAX2 (Addgene, #12260), and VSV- G envelope expressing plasmid pMD2.G (Addgene, #12259). .. After 48 hr, the lentiviruses were used for infecting A549 cells and then selected cells with puromycin (Thermo Fisher Scientific).

    Article Title: Beta-Catenin Mutations Can Impact on the Interplay Between Tumor and Immune Cells and Hepatic Microbiota in Hepatocellular Cancer
    Article Snippet: β-catenin (S33Y) -pcw107-V5 was a gift from Dr. David Sabatini and Kris Wood (Addgene plasmid #64616), and pcw107 was a gift from Dr. John Doench and David Sabatini (Addgene plasmid #62511). .. HEK 293T cells (3 × 106 cells) were seeded onto a 100 mm dish, and co-transfected with 3 μg of transfer expression plasmid DNA (mutant β-catenin lentivirus or empty lentivirus), 0.3 μg of VSV-G envelope expressing plasmid pMD2.G (gift from Dr. Didier Trono, Addgene plasmid #12259) and 2.7 μg of packaging plasmid psPAX2 (gift from Dr. Didier Trono, Addgene plasmid #12260) using Lipofectamine 2000 (Invitrogen, cat no. 11668030, Carlsbad, CA, USA). ..

    Article Title: Interaction between HIV-1 Tat and EBV Zta favours immune escape of B cells by downregulating HLA-ABC expression
    Article Snippet: .. HEK 293 T cells were transfected with second-generation lentiviral packaging plasmid psPAX2 (Addgene, # 12260), VSV-G envelope expressing plasmid pMD2.G (Addgene, # 12259) and transfer plasmid (FUdeltaGW-rtTA, FUW-tetO-YFP, FUW-tetO-YFP-Tat, FUW-tetO-YFP-Zta, FUW-tetO-YFP1-Tat-P2A-YFP2-Zta) at 15:6:20 ratio using Turbofect transfection reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions (20 μg of plasmid DNA and 40 μl of Turbofect transfection reagent were premixed in 2 ml of OptiMEM medium (Gibco) and added to 20 ml of DMEM/10% FBS cell medium in T-175 flask). ..

    Article Title: ORMDL3 restrains type I interferon signaling and anti-tumor immunity by promoting RIG-I degradation
    Article Snippet: .. HEK293T cells were co-transfected with lentiviral expressing plasmid, lentiviral packaging plasmid psPAX2 (Addgene, #12260), and VSV-G envelope expressing plasmid pMD2.G (Addgene, #12259). .. After 48 hr, the lentiviruses were used for infecting A549 cells and then selected cells with puromycin (Thermo Fisher Scientific).

    Article Title: Characterization of the p38α MAPK allosteric inhibition by a single chain Fv antibody
    Article Snippet: The all-in-one autoinducible lentiviral vector pCLX-pTF-R1-DEST-R2-EBR65 (gift from Patrick Salmon; Addgene plasmid # 45952; http://n2t.net/addgene:45952 ; RRID:Addgene_45952) containing a TET-inducible promoter was used to clone B2 and G9 anti-p38α scFv genes in frame with the GFP gene and a NLS tag sequence. .. Lentiviral particles were produced by co-transfection of the pCLX-scFv-GFP-NLS vector with the lentiviral packaging plasmid psPAX2 (gift from Didier Trono; Addgene plasmid # 12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260) and the VSV-G envelope expressing plasmid pMD2.G (gift from Didier Trono; Addgene plasmid # 12259; http://n2t.net/addgene:12259 ; RRID:Addgene_12259) into HEK293 cells as previously described . ..

    Article Title: Targeting a super-enhancer induced aldehyde dehydrogenase metabolic loop mitigates CDK4/6 inhibitor resistance in estrogen receptor-positive cancers.
    Article Snippet: .. In brief, the lentiviral expression plasmid (ALDH1A1 plasmid, K193Q/R-mutant ALDH1A1 plasmid and empty vector), packaging plasmid psPAX.2 (Addgene, Cat# 12260) and VSV-G envelope expressing plasmid pMD2.G (Addgene, Cat# 12259) were co-transfected into HEK293T cells. .. After 48 hours, the lentiviruses were harvested to infect cancer cells and then selected with puromycin (2 μg/ml) (Thermo Fisher Scientific) for 4 days.

    Article Title: The RNA-Binding Protein HuR Regulates Splicing Programs in Osteocytes
    Article Snippet: Plasmids were then extracted using Nucleospin plasmid DNA purification kit (Macherey-Nagel, Düren, Germany) followed by validating the insertions using colony-PCR confirming sequences by Sanger Sequencing (Eurofins Genomics, Tokyo, Japan). .. HEK293T cells were co-transfected with shRNA plasmids, lentiviral packaging plasmid psPAX2 (Addgene, Plasmid#12260) and VSV-G envelope expressing plasmid pMD2.G (Addgene, Plasmid#12259) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Distinct Sox9 single-molecule dynamics characterize adult differentiation and fetal-like reprogrammed states in intestinal organoids
    Article Snippet: .. For the production of lentivirus to deliver POI-tag transgenes into organoids, the third generation lentiviral pHAGE vector originally developed in the lab of Richard Mulligan was used together with the second generation lentiviral packaging plasmid psPAX2 (gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260)) and the VSV-G envelope expressing plasmid pMD2.G (gift from Didier Trono (Addgene plasmid #12259; http://n2t.net/addgene:12259 ; RRID:Addgene_12259)). ..

    Plasmid Preparation:

    Article Title: ORMDL3 restrains type-I interferon signaling and anti-tumor immunity by promoting RIG-I degradation
    Article Snippet: .. HEK293T cells were co- transfected with lentiviral expressing plasmid, lentiviral packaging plasmid psPAX2 (Addgene, #12260), and VSV- G envelope expressing plasmid pMD2.G (Addgene, #12259). .. After 48 hr, the lentiviruses were used for infecting A549 cells and then selected cells with puromycin (Thermo Fisher Scientific).

    Article Title: Beta-Catenin Mutations Can Impact on the Interplay Between Tumor and Immune Cells and Hepatic Microbiota in Hepatocellular Cancer
    Article Snippet: β-catenin (S33Y) -pcw107-V5 was a gift from Dr. David Sabatini and Kris Wood (Addgene plasmid #64616), and pcw107 was a gift from Dr. John Doench and David Sabatini (Addgene plasmid #62511). .. HEK 293T cells (3 × 106 cells) were seeded onto a 100 mm dish, and co-transfected with 3 μg of transfer expression plasmid DNA (mutant β-catenin lentivirus or empty lentivirus), 0.3 μg of VSV-G envelope expressing plasmid pMD2.G (gift from Dr. Didier Trono, Addgene plasmid #12259) and 2.7 μg of packaging plasmid psPAX2 (gift from Dr. Didier Trono, Addgene plasmid #12260) using Lipofectamine 2000 (Invitrogen, cat no. 11668030, Carlsbad, CA, USA). ..

    Article Title: Interaction between HIV-1 Tat and EBV Zta favours immune escape of B cells by downregulating HLA-ABC expression
    Article Snippet: .. HEK 293 T cells were transfected with second-generation lentiviral packaging plasmid psPAX2 (Addgene, # 12260), VSV-G envelope expressing plasmid pMD2.G (Addgene, # 12259) and transfer plasmid (FUdeltaGW-rtTA, FUW-tetO-YFP, FUW-tetO-YFP-Tat, FUW-tetO-YFP-Zta, FUW-tetO-YFP1-Tat-P2A-YFP2-Zta) at 15:6:20 ratio using Turbofect transfection reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions (20 μg of plasmid DNA and 40 μl of Turbofect transfection reagent were premixed in 2 ml of OptiMEM medium (Gibco) and added to 20 ml of DMEM/10% FBS cell medium in T-175 flask). ..

    Article Title: ORMDL3 restrains type I interferon signaling and anti-tumor immunity by promoting RIG-I degradation
    Article Snippet: .. HEK293T cells were co-transfected with lentiviral expressing plasmid, lentiviral packaging plasmid psPAX2 (Addgene, #12260), and VSV-G envelope expressing plasmid pMD2.G (Addgene, #12259). .. After 48 hr, the lentiviruses were used for infecting A549 cells and then selected cells with puromycin (Thermo Fisher Scientific).

    Article Title: Characterization of the p38α MAPK allosteric inhibition by a single chain Fv antibody
    Article Snippet: The all-in-one autoinducible lentiviral vector pCLX-pTF-R1-DEST-R2-EBR65 (gift from Patrick Salmon; Addgene plasmid # 45952; http://n2t.net/addgene:45952 ; RRID:Addgene_45952) containing a TET-inducible promoter was used to clone B2 and G9 anti-p38α scFv genes in frame with the GFP gene and a NLS tag sequence. .. Lentiviral particles were produced by co-transfection of the pCLX-scFv-GFP-NLS vector with the lentiviral packaging plasmid psPAX2 (gift from Didier Trono; Addgene plasmid # 12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260) and the VSV-G envelope expressing plasmid pMD2.G (gift from Didier Trono; Addgene plasmid # 12259; http://n2t.net/addgene:12259 ; RRID:Addgene_12259) into HEK293 cells as previously described . ..

    Article Title: Targeting a super-enhancer induced aldehyde dehydrogenase metabolic loop mitigates CDK4/6 inhibitor resistance in estrogen receptor-positive cancers.
    Article Snippet: .. In brief, the lentiviral expression plasmid (ALDH1A1 plasmid, K193Q/R-mutant ALDH1A1 plasmid and empty vector), packaging plasmid psPAX.2 (Addgene, Cat# 12260) and VSV-G envelope expressing plasmid pMD2.G (Addgene, Cat# 12259) were co-transfected into HEK293T cells. .. After 48 hours, the lentiviruses were harvested to infect cancer cells and then selected with puromycin (2 μg/ml) (Thermo Fisher Scientific) for 4 days.

    Article Title: The RNA-Binding Protein HuR Regulates Splicing Programs in Osteocytes
    Article Snippet: Plasmids were then extracted using Nucleospin plasmid DNA purification kit (Macherey-Nagel, Düren, Germany) followed by validating the insertions using colony-PCR confirming sequences by Sanger Sequencing (Eurofins Genomics, Tokyo, Japan). .. HEK293T cells were co-transfected with shRNA plasmids, lentiviral packaging plasmid psPAX2 (Addgene, Plasmid#12260) and VSV-G envelope expressing plasmid pMD2.G (Addgene, Plasmid#12259) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Distinct Sox9 single-molecule dynamics characterize adult differentiation and fetal-like reprogrammed states in intestinal organoids
    Article Snippet: .. For the production of lentivirus to deliver POI-tag transgenes into organoids, the third generation lentiviral pHAGE vector originally developed in the lab of Richard Mulligan was used together with the second generation lentiviral packaging plasmid psPAX2 (gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260)) and the VSV-G envelope expressing plasmid pMD2.G (gift from Didier Trono (Addgene plasmid #12259; http://n2t.net/addgene:12259 ; RRID:Addgene_12259)). ..

    Mutagenesis:

    Article Title: Beta-Catenin Mutations Can Impact on the Interplay Between Tumor and Immune Cells and Hepatic Microbiota in Hepatocellular Cancer
    Article Snippet: β-catenin (S33Y) -pcw107-V5 was a gift from Dr. David Sabatini and Kris Wood (Addgene plasmid #64616), and pcw107 was a gift from Dr. John Doench and David Sabatini (Addgene plasmid #62511). .. HEK 293T cells (3 × 106 cells) were seeded onto a 100 mm dish, and co-transfected with 3 μg of transfer expression plasmid DNA (mutant β-catenin lentivirus or empty lentivirus), 0.3 μg of VSV-G envelope expressing plasmid pMD2.G (gift from Dr. Didier Trono, Addgene plasmid #12259) and 2.7 μg of packaging plasmid psPAX2 (gift from Dr. Didier Trono, Addgene plasmid #12260) using Lipofectamine 2000 (Invitrogen, cat no. 11668030, Carlsbad, CA, USA). ..

    Produced:

    Article Title: Characterization of the p38α MAPK allosteric inhibition by a single chain Fv antibody
    Article Snippet: The all-in-one autoinducible lentiviral vector pCLX-pTF-R1-DEST-R2-EBR65 (gift from Patrick Salmon; Addgene plasmid # 45952; http://n2t.net/addgene:45952 ; RRID:Addgene_45952) containing a TET-inducible promoter was used to clone B2 and G9 anti-p38α scFv genes in frame with the GFP gene and a NLS tag sequence. .. Lentiviral particles were produced by co-transfection of the pCLX-scFv-GFP-NLS vector with the lentiviral packaging plasmid psPAX2 (gift from Didier Trono; Addgene plasmid # 12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260) and the VSV-G envelope expressing plasmid pMD2.G (gift from Didier Trono; Addgene plasmid # 12259; http://n2t.net/addgene:12259 ; RRID:Addgene_12259) into HEK293 cells as previously described . ..

    shRNA:

    Article Title: The RNA-Binding Protein HuR Regulates Splicing Programs in Osteocytes
    Article Snippet: Plasmids were then extracted using Nucleospin plasmid DNA purification kit (Macherey-Nagel, Düren, Germany) followed by validating the insertions using colony-PCR confirming sequences by Sanger Sequencing (Eurofins Genomics, Tokyo, Japan). .. HEK293T cells were co-transfected with shRNA plasmids, lentiviral packaging plasmid psPAX2 (Addgene, Plasmid#12260) and VSV-G envelope expressing plasmid pMD2.G (Addgene, Plasmid#12259) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA). ..



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